Fig 1: Markers of hepatic function, inflammation and fibrosis. a After 16 weeks ALAT-levels were significantly higher in NASH-fed rats compared to Control-, HFD- and HFr-fed rats. b Plasma levels of ASAT were higher in the NASH-group compared to both Control and HFr-groups. c Rats on NASH, HFD and HFr-diet had increased hepatic levels of MCP-1 compared to Control. d Hepatic TNF-α levels were significantly increased in the HFD fed rats compared to Control. e Haptoglobin was significantly increased in plasma of NASH-fed rats compared to rats fed Control, HFD and HFr. f Plasma TIMP-1 was significantly increased in the NASH-fed rats, compared to all other groups. Statistical significance: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Results are shown as mean ± SEM
Fig 2: Effect of high salt (HS), l‐buthionine‐sulfoximine (BSO) and resveratrol (R) on renal inflammatory markers. Vehicle (V) rats were kept on tap water. The levels of (A) interleukin 1β (IL‐1β), (B) interleukin 6 (IL‐6), (C) tissue necrosis factor α (TNF‐α), (D) monocyte chemoattractant protein 1 (MCP‐1), (E) granulocyte macrophage colony‐stimulating factor (GM‐CSF), and (F) interleukin 10 (IL‐10) were measured in renal proximal tubules after 6‐wk treatment by commercially available kits. Experiments were performed in quadruplicate (n=6–8 rats). *P<0.05 vs V by 1‐way ANOVA followed by a post hoc Newman–Keuls test.
Fig 3: QRHX inhibits PI3K/Akt signal pathway and down-regulates the level of inflammatory cytokines. (A) Inflammatory factor levels of MCP-1, IL-17A, TNF-a and IL-1ß. (B) Protein expression levels of p-PI3K, PI3K, p-Akt and Akt. (C) Quantitative analysis of p-PI3K, PI3K, p-Akt and Akt using ImageJ software. Data were expressed as mean ± SEM. **p < 0.01 vs. Normal group, #p < 0.05 and ##p < 0.01 vs. Model group.
Supplier Page from Abcam for Rat MCP1 ELISA Kit (CCL2)